Radiation Damage on Thaumatin: A Case Study of Crystals That Are Larger Than the Microfocusing X-ray Beam

Microfocusing X-rays direct high-density photons on crystal samples and can enhance the diffraction limit quality of collected data. However, these intense cause radiation damage to sample, which often results in undesirable structural information. Accordingly, a data collection strategy that minimizes is critical obtaining accurate In this study, single-point was investigated at two different X-ray exposure times (1 s 100 ms) using microfocusing thaumatin larger than beam. The statistics showed intensity Bragg peak did not gradually decrease until rotation reached 180°, it significantly decreased after exceeding value. Thaumatin structures exposed for 1 (Thaumatin1s) ms (Thaumatin100ms) were determined 1.13 Å resolution. temperature factors Asp60, Arg119, Lys163, Lys187 increased by damage. Specific observed disulfide bond Thaumatin1s but negligible Thaumatin100ms. Splitting reprocessing Thaumatin100ms electron density maps with minimal be obtained when satisfy completeness, I/sigma, CC1/2 parameters. These expand our understanding phenomena macromolecules used applications.